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Behavioral Vision Testing Using an Automated Observational Tracking System (VisioBox) to Obtain a Measure of Visual Acuity in Larval Zebrafish

Methods Mol Biol. 2026;3023:185-193. doi: 10.1007/978-1-0716-5198-8_13. ABSTRACT The optokinetic response (OKR) within zebrafish larvae consists of automatic stereotypical eye movements in response to a visual stimulus. This response is characterized by a tracking (slow phase) f…

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Methods Mol Biol. 2026;3023:185-193. doi: 10.1007/978-1-0716-5198-8_13.

ABSTRACT

The optokinetic response (OKR) within zebrafish larvae consists of automatic stereotypical eye movements in response to a visual stimulus. This response is characterized by a tracking (slow phase) followed by a rapid (fast phase) saccade in the opposite direction to the stimulus. Studying OKR behavior provides valuable information on the visual function of genetically modified animals. Here we describe a method for assessing visual function and color vision testing in zebrafish larvae at 5 days postfertilization.

PMID:42020910 | DOI:10.1007/978-1-0716-5198-8_13