<p><strong>Background:</strong> Despite the existence of vision screening standards, inconsistent implementation of these standards among optometrists persists.</p><p><strong>Aim:</strong> To evaluate the knowledge and practices of optometrists regarding vision screening standards specific to driver fitness assessments.</p><p><strong>Setting:</strong> Gauteng province, South Africa.</p><p><strong>Methods:</strong> A descriptive cross-sectional study employing a quantitative approach was conducted among optometrists via an online survey.</p><p><strong>Results:</strong> A total of 156 optometrists with a mean professional experience of 18.6 years ± 10 years participated in the study. The majority of optometrists (73%) demonstrated knowledge of the minimum vision screening standards required for driving. Male optometrists had higher odds of good knowledge compared to females (OR: 1.84 CI: 1.01 – 3.37, <em>p</em> = 0.048). Most optometrists (89%) adhered to these standards in their clinical practice. There was a significant association between knowledge level and the practice of measuring both visual acuity (VA) and visual fields (VF) (<em>χ</em><sup>2</sup> = 9.358, <em>p</em> = 0.025).</p><p><strong>Conclusion:</strong> While the majority of optometrists (73%) demonstrated knowledge of the minimum vision screening standard required for driving, 89% reported adherence to these standards in practice; it is concerning that 27% lacked sufficient knowledge, and 11% did not adhere to these screening standards.</p><p><strong>Contribution:</strong> The study highlights gaps in the knowledge and practice of vision screening standards among optometrists.</p>